Comparative Toxicity of Preservatives on Immortalized Corneal and Conjunctival Epithelial Cells
نویسندگان
چکیده
منابع مشابه
Comparative toxicity of preservatives on immortalized corneal and conjunctival epithelial cells.
PURPOSE Nearly all eye drops contain preservatives to decrease contamination. Nonpreservatives such as disodium-ethylene diamine tetra-acetate (EDTA) and phosphate-buffered saline are also regularly added as buffering agents. These components can add to the toxicity of eye drops and cause ocular surface disease. To evaluate the potential toxicity of these common components and their comparative...
متن کاملMucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines.
PURPOSE The corneal and conjunctival epithelia, which cover the ocular surface, play an important role in preventing pathogen penetrance into the eye and maintaining a wet-surface phenotype by producing highly hydrophilic mucin molecules for their apical surfaces. Ocular surface infections, wounding, and pathologies resulting in dry eye threaten sight and can cause blindness. Understanding the ...
متن کاملThe Corneal Epithelial Barrier and Its Developmental Role in Isolating Corneal Epithelial and Conjunctival Cells From One Another
Purpose During development, the corneal epithelium (CE) and the conjunctiva are derived from the surface ectoderm. Here we have examined how, during development, the cells of these two issues become isolated from each other. Methods Epithelia from the anterior eyes of chicken embryos were labeled with the fluorescent, lipophilic dye, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perc...
متن کاملCytochemical studies on endogenous peroxidase in conjunctival and corneal epithelial cells.
Endogenous peroxidase activity in conjunctival and corneal epithelial cells was studied cytochemically by the diaminobenzidine method. Many peroxidase-positive cells were found in the fornical conjunctiva. They were also seen, but less frequently, in the tarsal conjunctiva, limbus, and cornea. Three types of peroxidase-positive (cytochemically reactive) cells were differentiated besides goblet ...
متن کاملComparison of Ultra Structure and Gene Expression of Cultured Limbal Stem Cells and Fresh Conjunctival, Limbal and Corneal Tissues
Purpose: The present study intends to show the characteristics of cultured limbal stem cell (CLSCs) and to compare them with normal Conjunctival (C), Limbal (L) and Cornea (K) tissues. Materials and Methods: The expressions of a set of genes potentially involved in differentiation and stemness function of limbal stem cells were assessed in freshly prepared limbal, corneal, and conjunctival tis...
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ژورنال
عنوان ژورنال: Journal of Ocular Pharmacology and Therapeutics
سال: 2009
ISSN: 1080-7683,1557-7732
DOI: 10.1089/jop.2008.0098